They can be screened for:
- Sessile minimum inhibitory concentration assays (SMIC), inhibition or dispersal of the biofilm following treatment.
- Validated established robust reproducible pre-clinical multi-species interkingdom biofilm models (ranging from 2- 11 species) to support realistic antibiofilm efficacy prior to clinical assessment.
- Kill time for screening the efficacy of anti-microbial/anti-infective compounds.
- Quantitative compositional analysis of both live and total numbers of each species within mixed species biofilm over time during treatment ‑and a range of additional assays as requested.
- Established and Validated Biofilm Inhibition or Disruption Assay for Wound Dressings.
- A range of supporting imaging options including imaging of biofilms using scanning electron microscopy (SEM).
BluTest’s high quality biofilm testing model allows clients to test the antimicrobial efficacy of their products against microbial biofilms using a validated high-throughput testing platform. The assays are highly flexible and can be adapted to support the growth of different biofilm-forming microorganisms on 24, 96 and 384 well plates. Cell viability after treatment with the desired anti-microbial is then measured using a reliable metabolic assay.
Why test against biofilm
Up to 90% of bacteria exist as biofilms in nature and these can include combinations of bacteria, fungi, and yeast. Biofilms provide protection through mutualistic relationships between species and a surrounding extracellular matrix which can make them difficult to inhibit or disperse in addition to being less susceptible to anti-microbial agents.
The current standard testing methods of bactericidal and bacteriostatic actives uses planktonic bacteria. However, biofilms have been repeatedly shown to be significantly more resistant to anti-microbial compounds compared to their planktonic counterparts. As a result, such testing may misjudge the concentrations of active required to combat a biofilm.
Bespoke biofilm models
In addition to our standard testing Blutest has expertise in developing multi-species/ interkingdom biofilm models to suit a variety of real-world applications.
- Co-culture models containing biofilms and host cells for testing anti-inflammatory and anti-microbial actives. This model can be used to assess both the biofilm and cell response to treatment.
- Co-culture models to measure anti-inflammatory or protective effects of treatments in addition to any bactericidal or bacteriostatic properties. This model can be used to measure cell viability and a range of host immune responses via gene expression and protein release in addition to analysis on the biofilm itself.