Biofilm Testing

BluTest's Current Biofilm Testing Capabilities

  • MBIC (Minimum Biofilm Inhibitory Concentration)
  • Biofilm LD50 and LD90 Determinations
  • Biofilm Inhibition Assay
  • Established and Validated Biofilm Inhibition or Disruption Assay for Wound Dressings
  • Multi-species Biofilm Testing Platform
  • Product Development

Our assays are based on a simple and reproducible 96-well plate-based method originally designed for the formation of fungal biofilms and it's application to antifungal susceptibility testing.   

  • BluTest’s high quality biofilm testing model allows clients to test the antimicrobial efficacy of their products against microbial biofilms using a validated high-throughput testing platform. The assays are highly flexible and can be adapted to support the growth of different biofilm-forming microorganisms on 24, 96 and 384 well plates. Cell viability after treatment with the desired anti-microbial is then measured using a reliable metabolic assay. 

Commercial washes are more effective than azole antifungals against Candida albicans biofilms in vitro.

Biofilm formation by clinical isolates of Staphylococcus aureus.

WHY TEST AGAINST BIOFILM

  • Biofilms are of significant importance in medicine, dentistry, food processing, water supplies and industrial processes.  As biofilms are inherently resistant to currently used anti-microbials, the requirement for new and improved antibiotics, disinfectants and detergents is of chief concern.
  • BluTest can aid the discovery and improvement of anti-microbials by combining  biofilm testing technology with expert product development knowledge and experience.

Existing Biofilm Testing Panel

Microorganism                                                   Biofilm related problem

Candida albicans                                                Medical devices

Pseudomonas aeruginosa                                 Mucosal diseases, Water systems, Dentistry 

Staphylococcus aureus                                      Wound contamination, Medical devices 

Staphylococcus epidermidis                              Wound contamination

More information on biofilms can be sourced from: Are we any closer to beating the biofilm?: Novel methods of biofilm control.